Correlation of serum cartilage oligomeric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis: A pilot study in a Malaysian population.

Esha Das Gupta,Swan Sim Yeap,Wei Ren Ng,Abdul Kareem Bhurhanudeen,Shew Fung Wong,Mikko Juhani Lammi

doi:10.1371/journal.pone.0184802

Esha Das Gupta, Swan Sim Yeap + Show 4 more

Open Access

https://doi.org/10.1371/journal.pone.0184802

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Abstract

ObjectiveThe aim of this study was to investigate the correlations between serum cartilage oligomeric matrix protein (COMP), interleukin-16 (IL-16) and different grades of knee osteoarthritis (KOA) in Malaysian subjects.MethodsNinety subjects were recruited comprising 30 with Kellgren-Lawrence (K-L) grade 2 KOA, 27 with K-L grade 3 KOA, 7 with grade 4 KOA, and 30 healthy controls. All subjects completed the Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire. Serum COMP and IL-16 levels were measured using ELISA and their values log transformed to ensure a normal distribution.ResultsThere was no significant differences in levels of log serum COMP and IL-16 between healthy controls and KOA patients. There were no significant differences in the log serum COMP and IL-16 levels within the different K-L grades in the KOA patients. In KOA patients, log serum IL-16 levels significantly correlated with the WOMAC score (p = 0.001) and its subscales, pain (p = 0.005), stiffness (p = 0.019) and physical function (p<0.0001). Serum IL-16 levels were significantly higher in Malaysian Indians compared to Malays and Chinese (p = 0.024).ConclusionsIn this multi-ethnic Malaysian population, there was no difference in serum COMP and IL-16 levels between healthy controls and patients with KOA, nor was there any difference in serum COMP or IL-16 levels across the various K-L grades of KOA. However, there were significant inter-racial differences in serum IL-16 levels.

Highlights

In this multi-ethnic Malaysian population, there was no difference in serum cartilage oligomeric matrix protein (COMP) and IL-16 levels between healthy controls and patients with knee osteoarthritis (KOA), nor was there any differen
We set out to study serum COMP, a well-established marker in OA, in our multiethnic population to assess if its level was different in KOA patients compared to healthy controls, and if there was any difference in levels with KOA disease severity
In a systematic review and meta-analysis, serum COMP was found to be elevated in patients with radiographic knee OA compared to healthy controls and higher levels are associated with more severe disease [8]

Summary

Methods

Ninety subjects were recruited comprising 30 with Kellgren-Lawrence (K-L) grade 2 KOA, 27 with K-L grade 3 KOA, 7 with grade 4 KOA, and 30 healthy controls. All subjects completed the Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire. Serum COMP and IL-16 levels were measured using ELISA and their values log transformed to ensure a normal distribution. Data Availability Statement: All relevant data are within the paper and its Supporting Information files

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