Abstract
Objective To explore the correlation of serum c-peptide, soluble intercellular adhesion molecule-1 (sICAM-1), and NLRP3 inflammasome-related inflammatory factor interleukin-1β (IL-1β) after brain magnetic resonance imaging (MRI) examination with cerebral small vessel disease (CSVD). Methods A total of 72 CSVD patients treated in our hospital from December 2018 to December 2019 were selected as the case group and another 72 patients who presented cerebrovascular risk factors but obtained normal brain MRI examination result in the same period were selected as the control group. The serum specimen of patients in the two groups were collected, their serum c-peptide levels were measured by radio immunoassay, and their serum sICAM-1 and NLRP3 inflammasome-related inflammatory factor IL-1β were measured by enzyme-linked immunosorbent assay (ELISA), so as to analyze the correlation between these indicators and CSVD. Results Compared with the control group, the level values of serum c-peptide, sICAM-1, and IL-1β were significantly higher in the case group (P < 0.001), with CSVD being the dependent variable, and age, smoking, uric acid, history of stroke, serum c-peptide, sICAM-1, and IL-1β being the independent variables. A logistic regression analysis was conducted, and the result showed that age, smoking, serum c-peptide, sICAM-1, and IL-1β were the risk factors for CSVD, and by drawing the ROC curves, it could be concluded that the area under sICAM-1 curve was larger than that of other single indicator. Conclusion Elevation of level values of serum c-peptide, sICAM-1, and NLRP3 inflammasome-related inflammatory factor IL-1β is correlative with CSVD, and age, smoking, serum c-peptide, sICAM-1, and IL-1β are the independent risk factors for CSVD.
Highlights
Cerebral small vessel disease (CSVD) refers to the disease caused by small vessel lesion in the brain, which involves a vessel diameter of 30–800 μm [1, 2]
A total of 72 CSVD patients treated in our hospital from December 2018 to December 2019 were selected as the case group, and 72 patients who presented cerebrovascular risk factors but obtained normal brain magnetic resonance imaging (MRI) examination result in the same period were selected as the control group. e study met the World Medical Association Declaration of Helsinki (2013) [10]
In case of precipitation occurred during preservation, the blood samples should be centrifuged again for supernatant collection. e serum c-peptide level was measured by the radioimmunoassay with the kits purchased from Nanjing Xinfan Biotechnology Co., Ltd. e IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) levels were measured by the enzyme-linked immunosorbent assay (ELISA) method with the experiment steps in strict accordance with the specification on the kits. e kits were required to be preserved under the environment of 2–8°C before use; if use, the kits should be taken out from the cold storage environment and placed under the room temperature for 15–30 min before operation
Summary
Cerebral small vessel disease (CSVD) refers to the disease caused by small vessel lesion in the brain, which involves a vessel diameter of 30–800 μm [1, 2]. It is a brain wide dynamic disease, the exact pathogenesis of which has not been fully elucidated, and it is currently believed to be mainly related to hypoperfusion, microembolic embolism, ischemic damage, structural changes in small vessels, and some metabolic encephalopathies, such as homocysteinemia. Further studies have confirmed that the impairment of CSVD on cognitive function increases patients’ probability of developing dementia, undoubtedly illustrating the immense danger of the disease [4, 5].
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