Abstract
A correlation is presented for the partitioning of proteins in aqueous two-phase systems. The correlation relates the natural logarithm of the protein partition coefficient to the polyethylene glycol (PEG) concentration difference between the phases, ( w 1″ — w 1′), by the equation, 1n K = A( w 1″ — w 1′) + b( w 1″ — w 1′) 2. This relationship was shown to fit protein partitioning data obtained from eight two-phase sytems at pH 7.0, four consisting of PEG 8000—Dextran T-500—water systems and four consisting of PEG 3400—potassium phosphate—water systems.Seven different proteins, ribonuclease, lysozyme, trypsin, rhodanese, transferrin, hexokinase and invertase, with a molecular weight range of 10 000–270 000, were utilized in this work. Each of the proteins partitioned with different values of the empirical constants A and b.
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