Correlation of multi-drug resistance integron genes, blaESBL carriage of Extended-spectrum β-lactamas producing clinical isolates from equine with respiratory disorders

Abstract

The present study was aimed to identify phenotypical and genotypic antimicrobial characterization of clinical isolates obtained from equine with respiratory disorders in Egypt, as well as the correlation between the β-lactamases and integron genes in clinical isolates. Thirty two clinical isolates were identified as K.pneumoneae (15 isolates); P.aeruginosa (8 isolates); S.zooepidemicus (3 isolates) and S.equi (6 isolates). All isolates showed resistance to more than 3 classes of antibiotic and were considered as multidrug resistance isolates (MRD isolates), K. pneumoneae harbor ctx-M genes and shv-1 gene in highest rate (53.3% and 60% respectively). P.aeruginosa isolates harbor ctx-M genes and shv-1 gene (20% and 33.3% respectively) while S.equi and S.zooepidemicus harbor only ctx-M genes (33.3% and 20% respectively), tem-1 gene was observed in S. equi (66.7%) followed by K,pneumoneae (33.3%) and absent in P.aeruginosa. Significant correlation or association of intI genes with ctx-M genes in K. pneumoneae, P.aeruginosa and streptococcus species was observed, while it was significant with shv-1 in K.pneumoneae and streptococcus species. The significance was at p<0.05. It was concluded that the detection of integrons in most of the bacterial isolates promoters the phenomenon of in depth resistance because of these mobile genetic elements therefore the detection of those mobile gene cassettes in equine may be a keystone of multidrug resistance. Companion animals predominantly contain bla-ctx-M clusters, which might be animal origin, causing quick changes in drug-resistance epidemiology. ctx-M-1 and shv-1 also are commonly found among human pathogens signifying a standard clonal heredity and a possible ESBL dissemination source in farm and livestock.