Correlation of MDR1 and Anxa2 Overexpression with Breast Cancer Invasion and Metastasis

Abstract

Objective:To investigate the correlation between MDRl and Anxa2 mRNA expression and metastasis of breast cancer in large sample.Methods:Real-time reverse transcription-polymerase chain reaction(RT-PCR) was used to examine expression levels of MDRl and Anxa2 mRNA in 20 cases of primary ductal carcinoma in situ and normal breast tissues,as well as 100 cases of invasive ductal carcinoma,and 70 cases of normal tissue.Result:MDRl mRNA expression in ductal carcinoma in situ was significantly higher than that in the paired normal breast tissue(P=0.005).MDRl mRNA expression in invasive ductal carcinoma was significantly higher than that in ductal carcinoma(P=0.0051) and the normal breast cancer tissues(P=0.0196).No significant difference in Anxa2 mRNA expression was detected between ductal carcinoma in situ and the paired normal breast tissue.Anxa2 mRNA expression in invasive ductal carcinoma was significantly higher than that in ductal carcinoma(P=0.0008) and normal breast cancer(P0.0001).Overexpression of MDR1 and Anxa2 mRNA was correlated with lymph node metastasis(P0.01).MDR1 mRNA expression was also correlated with Anxa2 in invasive ductal carcinoma.Conclusion:MDR1 and Anxa2 overexpression are closely associated with the invasion and metastasis of breast cancer and the correlation between MDR1 and Anxa2 indicates a functional linkage between cancer metastasis and multidrug resistance during cancer progression.