Correlation of Intracellular Nucleotide Pools to Amino Acid Concentrations in Culture Media by the Application of Multivariate Methods

Abstract

Special ratios of intracellular nucleotide pools were shown to provide valuable information on the state of cultures of recombinant cell lines (Ryll and Wagner, 1992). In theory, these ratios could be used as control parameters in cultivation processes. However, as the determination of intracellular parameters is labor, time and cost intensive, this method can not be applied for routine process control. A strategy to overcome this restriction is to find more easily accessible parameters that can be reliably correlated to the intracellular nucleotide pools and ratios. Here we demonstrate in principle that this can be achieved by the application of multivariate methods such as principal component analysis (PCA) and partial least squares regression (PLS).