Abstract
The use of an IFN-γ ELISPOT assay to evaluate cellular immune responses has gained increasing popularity, especially as a surrogate measure for cytotoxic T lymphocyte (CTL) responses. We have compared the IFN-γ ELISPOT assay and the traditional51Cr release assay for detection of human natural killer (NK) cell activity. The cell populations used for evaluation of these assays included freshly isolated and IL-2-activated peripheral blood mononuclear cells (PBMC). CD56-positive cells were demonstrated to be the primary source of the IFN-γ signal when PBMC were evaluated with NK-sensitive targets in the IFN-γ ELISPOT assay. IFN-γ ELISPOT and51Cr release assays showed excellent correlation suggesting that NK activity can be reliably evaluated with methods other than the traditional51Cr release assays.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have