Abstract
BackgroundHuman kallikrein gene 6 (KLK6) is a member of the human kallikrein gene family (Kallikreins, KLKs). Human kallikrein-related peptidase 6 (hK6) is a trypsin-like serine protease encoded by the KLK6, has been reported to be highly expressed in several cancers including gastric cancer. In this study, we investigated the the correlation of hK6 expression with clinicopathological characteristics, tumor recurrence and prognosis in advanced gastric carcinoma after curative resection.MethodsWe retrospectively analyzed the clinical data of 129 cases advanced gastric cancer after curative gastrectomy. The expression of hK6 in advanced gastric cancer tissues compared to adjacent noncancerous tissues were examined, and the relationship between hK6 expression and clinicopathological characteristics was evaluated. In additional, these patients were followed up to investigate the relationship between hK6 expression and the survival time.ResultsThe positive rate of hK6 expression was significantly higher in advanced gastric cancer tissue, than that in adjacent noncancerous and gastric ulcer tissues (36.5%, 33.3%, respectively, P < 0.001). There was a close relationship between hK6 expression and TNM stage (P = 0.005), vascular invasion (P = 0.037) and perineural invasion (P = 0.035). Furthermore, patients with hK6 positive showed significantly higher recurrence and poorer prognosis than those with hK6 negative. Multivariate analysis showed that hK6 expression was a significant independent factor for tumor recurrence and overall survival.ConclusionhK6 is overexpressed in advanced gastric cancer tissues. Its clinical utility may be used as an unfavorable indicator in predicting tumor recurrence and prognosis for advanced gastric cancer after operation. This study also suggests that hK6 might be a potential therapeutic target for gastric cancer.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/8558403578787206
Highlights
Gastric cancer is one the most common malignancies and the second leading cause of death among all cancers in the clinical [1], and surgical resection remains currently the only treatment with curative
We evaluated the expression of Human kallikrein-related peptidase 6 (hK6) in the surgical specimens of advanced gastric cancer tissues, paired adjacent noncancerous tissues and gastric ulcer tissues using immunohistochemistry HRP, and analyzed their correlations with clinicopathological characteristics and patients survival to clarify the significance of hK6 in advanced gastric cancer after curative surgery
Tumor size was greater than 5 cm in 70 cases, and less than 5 cm in 59 cases, 16 cases classified as well or moderately differentiated, 113 cases as poorly differentiated, 19 cases were at T2, 55 cases were at T3 and T4 respectively
Summary
Gastric cancer is one the most common malignancies and the second leading cause of death among all cancers in the clinical [1], and surgical resection remains currently the only treatment with curative. KLK6 is a member of the KLKs, which encodes for human kallikrein-related peptidase 6 (hK6). It is a serine protease made up of 223 amino acids with trypsin-like activity. Anisowicz et al [3] first isolated the full-length cDNA of KLK6, named protease M, which is strongly expressed at the mRNA level in certain primary breast cancer cell lines and in ovarian cancer tissues and cell lines. Yamashiro et al [4] cloned this same gene, named neurosin, from a cDNA library prepared from a human colorectal cancer cell line (COLO 201). Human kallikrein-related peptidase 6 (hK6) is a trypsin-like serine protease encoded by the KLK6, has been reported to be highly expressed in several cancers including gastric cancer. We investigated the the correlation of hK6 expression with clinicopathological characteristics, tumor recurrence and prognosis in advanced gastric carcinoma after curative resection
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