Correlation of expressions of S100A8 and S100A9 and its prognostic potential in nasopharyngeal carcinoma

Wenbing Hu,Xi Chen,Jun Chen,Lei Gu,Zezhang Tao,Gangsheng Wang,Lun Ye

doi:10.4314/tjpr.v16i11.2

Wenbing Hu, Xi Chen + Show 5 more

Open Access

https://doi.org/10.4314/tjpr.v16i11.2

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Abstract

Purpose: To investigate the expressions of S100 calcium-binding proteins A8 (S100A8) and S100 A9 (S100A9) in nasopharyngeal carcinoma (NPC) tissues and their correlation with clinical pathological characteristics and prognosis of NPC.Methods: Ninety-two NPC tissue samples and 92 nasopharyngitis tissue samples (controls) were collected. All the NPC patients were on follow-up for more than 5 years. The expressions of S100A8 and S100A9 were determined in these tissues by immunohistochemistry. The relationship between the expressions of S100A8 and S100A9, and the clinico-pathological features were analyzed by Spearman correlation analysis, and the results evaluated by Kaplan-Meier method.Results: The differences between their expressions in the two tissues were statistically significant (p < 0.05). Neither gender nor age was associated with expressions of S100A8 and S100A9 (p > 0.05), but they were closely related to the degree of differentiation, clinical stages and metastasis of lymph node (p < 0.05). The expression of S100A8 had significant positive correlation with the expression of S100A9 (r = 0.393, p = 0.000). Prognosis of patients with positive expressions of S100A8 and S100A9 was poor, when compared to patients with negative expressions of these proteins (p < 0.05).Conclusion: Expressions of S100A8 and S100A9 are closely related to the development of NPC. High expressions of the two proteins may have an important influence in the progress of tumor invasion but are associated with poor prognosis of NPC. These findings could be significant indicators of early diagnosis, effectiveness of treatment and prognosis of NPC.Keywords: S100A8, S100A9, Calcium-binding proteins, Prognosis, Nasopharyngeal carcinoma, Prognosis

Highlights

Nasopharyngeal carcinoma (NPC) usually occurs on the top, or in the lateral wall of the nasopharynx
Measurement data are expressed as mean ± standard deviation (SD) and t test was used for the comparison between means
This is in agreement with the results obtained in the present study which showed that S100 calcium-binding proteins A8 (S100A8) and S100 A9 (S100A9) had closely correlation with the degree of differentiation, stage of TNM system and lymph node metastasis, indicating that the expressions of S100A8 and S100A9 increase with the degree of malignancy of NPC

Summary

INTRODUCTION

Nasopharyngeal carcinoma (NPC) usually occurs on the top, or in the lateral wall of the nasopharynx. It is a malignant head and neck tumor associated with high incidence and fatality rate [1,2]. Thereafter streptavidin-peroxidase (S-P) was added, and the complex was incubated for 45 min at 37 oC, and stained with BAD, again with hematoxylin. This positive tissue section was used as positive control, while in the negative control, the first antibody was replaced with citrate buffer.

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Conflict of Interest