Abstract
Objective To observe the changes of the blood pressure after intracerebral hemorrhage （ICH） in spontaneously hypertensive rats （SHRs） as well as the expressions of α2A-adrenergic receptor （AR） in center （brain tissue） and peripheral （renal tissue） αA-AR and to investigate the correlation between α1A-AR/α2A-AR and blood pressure regulation in acute hypertensive ICH. Methods A total of 30 six-month-old male SHRs were randomly divided into a sham-operation group and ICH （day 1,3, 7 and 14） groups （n =6 in each group）. Blood pressure was measured by the tail-cuff method. Collagenase Ⅳ was injected into caudate-putamen nucleus to induce a model of ICH. The expressions of α1A-AR and α2A-AR were detected by using immunohistochemistry and reverse-transcription polymerase chain reaction. Results One day after ICH, the blood pressure was 195.4 ± 8.39 mm Hg, and it was significantly higher than 177.8 ±8.69 mm Hg （P =0. 000） before ICH and 184. 1 ±3.76 mm Hg in the shamoperation group （P =0. 002）. At day 3 it was 185.3 ±9.22 mm Hg, and it was lower than that at day 1. It was 177.7 ±5.62 mm Hg and 176.7 ±6.06 mm Hg at day7 and 14 respectively, which almost returned to the normal level before ICH. The α1A-AR mRNA and protein in renal tissue at day 1 after ICH were 0.91 ± 0. 013 and 0. 944 ± 0. 142%, respectively. They were higher than 0.89 ±0. 018 and 0. 779 ±0. 103% in the sham-operation group, and they reached the peak （0.93 ± 0. 015, P = 0. 008; 1. 526 ± 0. 296%, P = 0. 010） at day 3. The α2A-AR mRNA and protein in brain tissue were 0.93 ± 0. 020 and 2.64 ± 0. 293 % at day 3 after ICH, and they were significantly higher than 0.86 ±0. 019 （P =0. 001） and 1. 070 ±0. 155% （P = 0. 020）, and 0.87 ±0. 029 （P =0. 000） at day 1 after ICH and 1. 629 ±0. 488% （P =0. 023） in the sham-operation group, The changes of blood pressure in the ICH day 1 to day 7 groups in SHRs and correlation coefficient of α2A-AR mRNA absorbance in brain tissue r was - 0. 509 （P = 0.031 ）, and the correlation coefficient of α2A-AR protein-expression volume fraction in brain tissue r was - 0. 473 （P = 0. 047）. Conclusions Regulation of blood pressure during acute ICH may have certain correlation with the up-expressions of α2A-AR in brain tissue and α1A-AR in renal tissue. Key words: intracerebral hemorrhage; hypertension; adrenoceptors; rat
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