Correlation of expression of 25-hydroxyvitamin D in peripheral blood and immune cell subsets of tuberculosis patients

Abstract

Objective This study aim to discover the relationship between serum 25-hydroxyvitamin D and tuberculosis patients, and we achieve new ideas for the prevention and treatment of tuberculosis by analyzed its correlation with body immunity cells. Methods Fifty-four patients diagnosed with tuberculosis who admitted to Suzhou Fifth People’s Hospital from December 2018 to May 2019 were included in the experimental group, and 60 healthy persons undergoing outpatient medical examinations in our hospital at the same period were selected as the control group. 25-hydroxyvitamin D levels were compared in two groups for discovering the relationship between 25-hydroxyvitamin D and tuberculosis. Spearman correlation was used to analyze the correlation of 25-hydroxyvitamin D with these levels of CD3 + , CD4 + , CD8 + and CD4 + /CD8 + ratio as well as other immune cells in patients with tuberculosis. Results The serum 25-hydroxyvitamin D level of TB patients 9.75 (5.1, 15.8) ng / mL was significantly lower than that of healthy persons 16.8 (6.8, 32.7) ng / mL ( P 0.05).The serum 25-hydroxyvitamin D level was significantly positively correlated with peripheral blood CD4 + level and CD4 + /CD8 + ratio ( r =0.317 2 and 0.404 4, both P 0.05) , 且血清 25-羟基 维生素 D 水平与外周血 CD4 + 、CD4 + /CD8 + 呈明显正相关 ( r =0.317 2、0.404 4, 均 P <0.05) , 与外周血 CD8 + 呈明显负相关 ( r =-0.329 9, P =0.015) ; 与 NK 细胞水平呈正相关 ( r =0.326 2, P =0.016), 与 B 淋巴细胞及单核细胞呈负相关 ( r =-0.333 9、-0.301 2, 均 P <0.05)。 结论 结核患者中血清 25-羟基维生素 D 明显降低, 可能参与了机体免疫功能调节。