CORRELATION OF DRUG RESISTANCE PATTERN WITH LIPASE PRODUCTION IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE

Abstract

Aim:The aim of our study is to correlate drug resistance patterns with Lipase production in clinical isolates of Klebsiella pneumoniae. Materials and Methods:Atotal of 150 clinical isolates of Klebsiella pneumoniae from various clinical samples collected during the period from July 2020 to December 2020 was included in this study. Lipase production was detected by tween 80 agar and an antibiotic susceptibility test has been performed by Kirby-Bauer disk diffusion method as per CLSI guidelines [10]. Extended Spectrum Beta-lactamase (ESBL) producers were detected by combined disc diffusion method and Carbapenemase production was detected by using the E-test strip method. Results and Discussion: Out of 150 consecutive non-duplicate isolates of Klebsiella pneumoniae, 74 isolates from Exudate, 7 from Blood, and 69 were from urine. Among 150 isolates, 85(56.6%) isolates produced Lipase production, 43(57.3%) were ESBL producers and 20(32.25%) isolates were positive for Carbapenemase production. Conclusion: Showing virulence in clinical isolates of Klebsiella pneumoniae, and the antibiotic susceptibility pattern with lipase production in Klebsiella pneumoniae were noticed. If the virulence is increased in Klebsiella pneumoniae, a drug-resistant pattern also shows more resistance in number. And nally, to correlate the ESBL and Carbapenemase producer with lipase production, in that also the ESBL and Carbapenemase producer shows more number with lipase production. And also noted, when compared with outpatients, inpatients show more lipase production. And hence ampicillin is an intrinsic resistance to Klebsiella pneumoniae. In this study, to correlate the drug-resistant pattern, ESBL, and carbapenemase producer with lipase production in clinical isolates of Klebsiella pneumoniae.