Correlation of Chemical Urinalysis to Microscopic Urinalysis and Urine Culture: Implications for Reflex Urinalysis Workflows.

Abstract

Urinalysis (UA) reflex testing approaches, which offer potential for savings in labor and result turnaround time, may rely on the performance of a chemical UA screen to determine which urine samples need microscopic UA and/or urine culture. We correlated chemical UA, microscopic UA, and urine culture results to determine the performance of chemical UA as a screening tool for reflex testing approaches. Consecutive UA results for 9127 tests (simultaneous chemical UA and microscopic UA) were retrospectively reviewed and correlated. Urine culture results were also correlated for 3127 samples that had urine culture ordered within 24 h of UA. Positivity criteria for each UA method were predefined. Chemical UA yielded the following performance specifications for predicting microscopic findings: 93.0% sensitivity, 56.9% specificity, 64.7% positive predictive value, 90.5% negative predictive value. 3.2% of samples were negative by chemical UA but positive by microscopic UA. Of the samples with urine culture results available, 6.3% were negative by chemical UA but had clinically-significant positive urine cultures. Reflex testing of microscopic UA and/or urine culture dependent from chemical UA results provides a feasible opportunity to reduce unnecessary testing.