Abstract
Background: the difference in expression of type IV collagen in borderline tumors and ovarian carcinomas has been studied, but the association with adhesion molecules like CD44 have not gain enough interest. Objectives: The purpose of this study is to assess the expression of CD44v6 and type IV collagen status in borderline tumors and invasive ovarian carcinomas and the correlation between them to define the role of these molecules in tumor invasion and metastasis. Type of the study: A cross sectional study Methods: The study included a total of (101) formalin-fixed paraffin-embedded ovarian tissue blocks; of which (19) cases were borderline tumors and (82) cases were overt ovarian carcinomas. Sections from each block were immunohistochemically stained for CD44v6 and type IV collagen. Results: CD44v6 was significantly correlated with FIGO stage of borderline tumors (p=0.001) and ovarian carcinoma (<0.001) and with histological grade of ovarian carcinomas (p=0.004). There was significantly higher expression of type IV collagen in borderline tumors compared to invasive carcinoma(p<0.001) this significance was also seen in correlation to age, stage and grade of the invasive carcinoma, no significant differences in other clinicopathological features were found. There was negative correlation between CD44 v6 & type IV collagen which was statistically significance (P<0.05) in carcinoma but not in borderline tumors. Conclusions: Our data suggest that observed inverse correlation of type IV collagen expression with CD44v6 positivity in surface epithelial tumors indicates that these molecules may cooperate in the invasion and progression of ovarian carcinomas.
Highlights
Mobile 07808498577, e-mail: one of the most important roles of the pathologist is to distinguish borderline tumors from invasive ovarian carcinomas, this distinction is one of the most common problems in ovarian tumor pathology as these tumors have many controversial aspects, such as the histologic criteria for their diagnosis, their biologic behavior, their molecular and biologic relationship to frankly invasive tumors, and their clinical management [1]
Variants of CD44 have been proposed to be important in cancer invasion and metastasis and according to Zhonghua et al [13] the expression of CD44v6 is closely related to carcinoma metastasis and appears to be more suitable as a metastatic marker
The current study showed significant association between FIGO stages of ovarian carcinoma and CD44v6 immune expression, the expression were increased with higher stage of ovarian carcinoma (p
Summary
Mobile 07808498577, e-mail: one of the most important roles of the pathologist is to distinguish borderline tumors from invasive ovarian carcinomas, this distinction is one of the most common problems in ovarian tumor pathology as these tumors have many controversial aspects, such as the histologic criteria for their diagnosis, their biologic behavior, their molecular and biologic relationship to frankly invasive tumors, and their clinical management [1]. It is known that proteolytic enzymes may facilitate the breakdown of type IV collagen, which is a major component of basement membranes, and its degradation is an essential event in tumor cell invasion [8]. The aim of this study is to assess the expression of CD44v6 in relation to type IV collagen in ovarian borderline and malignant surface epithelial tumors to identify any role in invasion and metastasis of ovarian carcinoma and correlation of these markers with clinicopathological parameters. One representative (4 micrometer) section was stained with Hematoxylin and Eosin and the histopathological diagnosis was revised, while two (4 micrometer) sections were placed on positively charged slides and stained immunohistochemically using three steps- indirect streptavidin method for monoclonal mouse antibodies including anti CD44v6 antibody, clone (VFF-7) and monoclonal Mouse Anti-Collagen IV antibody, both manufactured by Abcam. CD44v6 : Brown membranous & / or cytoplasmic staining pattern of epithelial cells even if staining was focal in tumor cell
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