Correlation of CD24 expression with histological grading and TNM staging of retinoblastoma.

Syed Muhammad Ishaque

doi:10.12669/pjms.321.8828

Abstract

Objective:Correlation of CD24 expression with histological grading and TNM staging of retinoblastoma.Methods:This cross-sectional study was conducted in the Department of Pathology, BMSI, JPMC and NICH from 1st January 2009 to 31st December 2013. A total 68 diagnosed cases of retinoblastoma were selected for CD24 immuno staining. The data was analyzed by using SPSS version 22.Results:Out of 68 cases 7.35% showed grade 1 followed by 11.76% in G2, 26.47% in G3 and 54.41% in G4. Majority of cases i.e. 60.29% in stage IV followed by 19.11% in stage I, 10.29% each in stage II and stage III. CD24 immuno staining positivity was seen in majority of grade 3 and grade 4. In grade 3, 38.88% showed moderate and 22.22% strong immuno reaction. Amongst grade 4, 40.54% showed moderate and 13.51% strong positive. Variable immuno pattern was observed according to TNM staging. In stage I, 46.15% showed moderate and 7.69% strong positivity, while in stage II, 57.14% were negative for saining. In stage III, 42.85% were negative while 28.57% each showed moderate and strong staining. Majority of cases in stage IV i.e. 48.78% were negative for staining while 34.14%, 17.07% showed moderate and severe CD24 immuno staining.Conclusion:Majority of grade I retinoblastoma were in TNM stage I & II and mostly were immuno negative. Lymph node and distant metastatic cases were 75% in G4 and 25% in G3, all of them showed moderate to strong immunoreactivity. These results showed that CD24 expression may be a marker of poor prognosis in retinoblastoma. Whereas TNM staging of retinoblastomas with CD24 expression had varying pattern and showed no significant correlation between them.

Highlights

IntroductionRetinoblastoma is a highly malignant tumor, originating from primitive and immature cells of neural retina and is considered the most common intraocular primary childhood malignancy.[1,2,3,4]
Retinoblastoma is a highly malignant tumor, originating from primitive and immature cells of neural retina and is considered the most common intraocular primary childhood malignancy.[1,2,3,4]Worldwide 5,000 new cases of retinoblastoma are diagnosed annually
Out of 68 selected cases majority i-e 60.29% were in Stage IV, 10.29% each were in Stage III and Stage II, while 19.11% were in Stage

Summary

Introduction

Retinoblastoma is a highly malignant tumor, originating from primitive and immature cells of neural retina and is considered the most common intraocular primary childhood malignancy.[1,2,3,4]. Worldwide 5,000 new cases of retinoblastoma are diagnosed annually. Incidence ranges from 1 in 15,000 to 1 in 18,000 live birth and accounts for 2 to 4% of all childhood malignant neoplasm.[5,6] In US its incidence is 11.8 per million under 4 years of age.[6] African countries showed higher incidence of retinoblastoma accounting for 10 to15%. North America, Australia and Asian sub continent accounts for 2 to 4% of neoplasm in children.[7,8,9]

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