Correlation of Absorbance Changes and Thylakoid Fusion with the Induction of Oxygen Evolution in Bean Leaves Greened by Brief Flashes

Abstract

Dark-grown bean leaves (Phaseolus vulgaris) which had been greened for several days in a repetitive series of brief xenon flashes were studied during the initial induction period when O(2) evolution first appears. The induction of O(2) evolution requires actinic irradiation (e.g. 2 mw/cm(2) of red light) and goes to completion in about 8 minutes with a half-time just under 3 minutes. Absorbance measurements on the intact leaves showed that a change of a carotenoid pigment, monitored at 505 nm, was closely correlated with the rate of O(2) evolution during the induction period. Inhibitor studies, however, showed that the absorbance change persisted in the presence of a number of inhibitors which blocked O(2) evolution. Electron microscopy revealed that the primary thylakoids which were unfused in the flashed leaves before induction became fused in pairs or groups of three during the 8-minute induction period. It is postulated that the 505-nm absorbance change of the carotenoid pigment is correlated more directly with the fusion process than with O(2) evolution. Heat treatment (45 C for 5 min) or infiltration with 0.8 m tris, which prevented the fusion process, also prevented the absorbance change.If the leaves were preilluminated for 8 minutes with very weak red light (20 muw/cm(2)) which induced no O(2) evolution, absorbance change, or thylakoid fusion, there was an immediate burst of O(2) evolution at the onset of actinic irradiation and the induction period, as noted by O(2) evolution or by the 505-nm absorbance change, was reduced to 2 minutes (half-time of 40 seconds). It is concluded that the electron transport system in the flashed leaves is blocked at the Mn site between water and photosystem II and that the photoactivation of Mn into the thylakoid membranes occurs during the low light, photoactivation process. After the electron transport chain is thus repaired, ion-pumping mechanisms driven by actinic light may lead to steady-state photosynthesis as well as to thylakoid fusion.