Correlation between virulence and genetic structure of Escovopsis strains from leaf-cutting ant colonies in Costa Rica.

Abstract

Leaf-cutting ants (genera Atta and Acromyrmex) cultivate a specialized fungus for food in underground chambers employing cut plant material as substrate. Parasitism occurs in this agricultural system and plays an important role in colony fitness. The microfungi Escovopsis, a specialized mycoparasite of the fungal cultivar, is highly prevalent among colonies. In this study, we tested the antagonistic activity of several Escovopsis strains from different geographical areas in Costa Rica. We employed a combination of laboratory tests to evaluate virulence, including pure culture challenges, toxicity to fungus garden pieces and subcolony bioassays. We also performed a phylogenetic analysis of these strains in order to correlate their virulence with the genetic structure of this population. The bioassays yielded results consistent between each other and showed significant differences in antagonistic activity among the parasites evaluated. However, no significant differences were found when comparing the results of the bioassays according to the source of the ants' fungal cultivar. The phylogenetic analyses were consistent with these results: whilst the fungal cultivar phylogeny showed a single clade with limited molecular variation, the Escovopsis phylogeny yielded several clades with the most virulent isolates grouping in the same well-supported clade. These results indicate that there are Escovopsis strains better suited to establish their antagonistic effect, whilst the genetic homogeneity of the fungal cultivars limits their ability to modulate Escovopsis antagonism. These findings should be taken into consideration when evaluating the potential of Escovopsis isolates as biocontrol agents for this important agricultural pest in the Neotropics.