Correlation between variants of leukotriene A4 hydrolase gene and risk of ischemic stroke in population of southern Zhejiang Province

Abstract

Objective To investigate the correlation between two single nucleotide polymorphisms of the leukotriene A4 hydrolase (LTA4H) gene(rs2660845 and rs2540493) and risk of ischemic stroke in population of southern Zhejiang Province. Methods A total of 300 ischemic stroke patients and 300 healthy controls, recruited from the Department of Neurology, Third Affiliated Hospital of Wenzhou Medical University between September 2010 and June 2013, were enrolled in this study. Two single nucleotide polymorphisms of the LTA4H gene (rs2660845 and rs2540493) were analyzed by polymerase chain reaction and matrix-assisted laser desorption/ionization time of flight, respectively. Sixty-seven patients and thirty controls were randomly selected (complete randomization) and detected the serum leukotriene B4 (LTB4) concentration by ELISA method. Results There was no evidence of association between the two variants of LTA4H gene and the risk of ischemic stroke or its TOAST (Trial of Org 10 172 in acute stroke treatment) subtypes (P>0.05). Analysis of LTB4 levels revealed that there was no statistically significant difference in serum LTB4 concentration between patients (n=67) and controls (n=30; 0.991±0.305 vs 1.035±0.498; P=0.692), and no statistically significant difference in LTB4 concentration was found among the three genotypes of rs2660845 as well (AG genotype vs AA genotype vs GG genotype: 0.938±0.269 vs 1.038±0.268 vs 1.043±0.383; P=0.401). Conclusion The present study suggests that there is no association between the two polymorphisms in the LTA4H gene and risk of ischemic stroke in population of southern Zhejiang Province. Key words: Brain ischemia; Stroke; Epoxide hydrolases; Polymorphism, single nucleotide; Mutation