Abstract
Numerous studies have shown the presence of DNA strand breaks in human ejaculated spermatozoa using a number of different assays. The two commonly used tests to measure sperm DNA damage are the sperm chromatin structure assay (SCSA) and the TUNEL assay. However, little is known about the correlation between assays and the inter-lab variability of these assays. We thought to validate the TUNEL assay, examine the correlation between sperm chromatin structure assay (SCSA) and the TUNEL assay and the inter-lab variability of the TUNEL assay.
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