Abstract
The Human Zona Pellucida (ZP) is composed of three concentric layers that differ in the retardance and azimuth of ZP filaments and can be imaged by polarization microscopy. Retardance is an optical propriety of highly ordered molecules, which can be measured by polarization microscopy. Changes in the morphological characteristics of the ZP in relation to age have not yet been fully described, however previous reports suggest age effects on zona structure and function. The objective of this study was to test the hypothesis that the morphology and architecture of the ZP changes with age.
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