Correlation between the Antibiotic Resistance Genes and Susceptibility to Antibiotics among the Carbapenem-Resistant Gram-Negative Pathogens.

Salma M Abdelaziz,Ibrahim S Yahia,Nadia A Hassouna,Mahmoud A Yassien,Khaled M Aboshanab

doi:10.3390/antibiotics10030255

Salma M Abdelaziz, Ibrahim S Yahia + Show 3 more

Open Access

https://doi.org/10.3390/antibiotics10030255

Copy DOI

Journal: Antibiotics	Publication Date: Mar 4, 2021
Citations: 12	License type: CC BY 4.0

Affiliation: Ain Shams University, King Khalid University

Abstract

In this study, the correlation between the antibiotic resistance genes and antibiotic susceptibility among the carbapenem-resistant Gram-negative pathogens (CRGNPs) recovered from patients diagnosed with acute pneumonia in Egypt was found. A total of 194 isolates including Klebsiella pneumoniae (89; 46%), Escherichia coli (47; 24%) and Pseudomonas aeruginosa (58; 30%) were recovered. Of these, 34 (18%) isolates were multiple drug resistant (MDR) and carbapenem resistant. For the K. pneumoniae MDR isolates (n = 22), blaNDM (14; 64%) was the most prevalent carbapenemase, followed by blaOXA-48 (11; 50%) and blaVIM (4; 18%). A significant association (p value < 0.05) was observed between the multidrug efflux pump (AcrA) and resistance to β-lactams and the aminoglycoside acetyl transferase gene (aac-6’-Ib) gene and resistance to ciprofloxacin, azithromycin and β-lactams (except for aztreonam). For P. aeruginosa, a significant association was noticed between the presence of the blaSHV gene and the multidrug efflux pump (MexA) and resistance to fluoroquinolones, amikacin, tobramycin, co-trimoxazole and β-lactams and between the aac-6’-Ib gene and resistance to aminoglycosides. All P. aeruginosa isolates (100%) harbored the MexAB-OprM multidrug efflux pump while 86% of the K. pneumoniae isolates harbored the AcrAB-TolC pump. Our results are of great medical importance for the guidance of healthcare practitioners for effective antibiotic prescription.

Highlights

Gram-negative bacteria pose a significant treatment challenge to medical staff due to their widespread resistance to antibiotics
The Gram-negative isolates collected in this study (n = 194) were K. pneumoniae (46%), E. coli (24%) and P. aeruginosa (30%)
A total of 194 clinical Gram-negative bacterial isolates were recovered from sputum clinical specimens discharged from the microbiology laboratory at Al-Demerdash Hospital, Cairo, Egypt from patients suffering from acute lobar pneumonia according to hospital records during the period from January 2018 to February 2019

Summary

Introduction

Gram-negative bacteria pose a significant treatment challenge to medical staff due to their widespread resistance to antibiotics. Klebsiella pneumoniae is a frequent human pathogen that causes many diseases, such as pneumonia, urinary tract infections and surgical wound infections, and serious life-threatening infections, such as endocarditis and septicemia. It can cause necrotizing pneumonia and pyogenic liver abscesses [1]. Escherichia coli, which is another member of the Enterobacteriaceae family, is the most common commensal in the gastrointestinal tract of people as well as an important pathogen It can cause several diseases, including watery diarrhea, bloody diarrhea, urinary tract infections, acute neonatal meningitis and sepsis [4]

Objectives

Methods

Results

Conclusion