Abstract
In mouse anococcygeus cells, simultaneous measurements of membrane currents and changes in intracellular Ca 2+ were obtained using “perforated-patch” whole-cell recordings and Fura-2 microfluorimetry. Carbachol (50 μM) and cyclopiazonic acid (10 μM) produced a biphasic inward current; a transient Ca 2+-dependent chloride current ( I ClCa), followed by a smaller, sustained current ( I DOC). This response was mirrored by a biphasic increase in the intracellular Ca 2+ concentration. SKF96365 (1-{β-[3-(4-methoxyphenyl) propoxyl]-4-methoxyphenethyl}-1 H-imidazole; 10 μM) and Cd 2+ (100 μM) inhibited both I DOC and the sustained increase in intracellular Ca 2+; La 3+ (400 μM) inhibited neither response. The results confirm that the non-selective cation current I DOC underlies capacitative Ca 2+ influx supporting sustained contractions in this tonic smooth muscle.
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