Abstract
SLC test with the mixed culture of the donor's skin and recipient's lymphocytes, was investigated for tissue typing in mice. Mice used were C3H/He (H-2k), CBA (H-2k) and (C3H/He × C57BL/6) F1. SLC test was carried out with a stationary monolayer culture in Eagle's basal medium containing 0.25% phytohemagglutinin-P for 72 hours. The extent of the reaction was estimated by both the frequency of the appearance of blast-like cells on morphological observation, and the number of the aggregated lymphocytes surrounding the skin in Ehrlich's sight. The extent of the blast formation and aggregation of lymphocytes was compared with the survival time of the skin grafts in various combinations of donors and recipients. A significant correlation between the result of SLC test and the transplant survival time was found.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.