Correlation between murine liver cytochrome p450 2b1 induction by halogenated hydrocarbons and toxicity

Abstract

The aim of this investigation was to study the relationship between the induction of cytochrome P450 2B1 isozymes by thirteen halogenated hydrocarbons and their toxicity. Each chemical, 1,1,2,2‐tetrabromoethane (1,1,2,2‐TBE), 1,1,1,2‐tetrachloroethane (1,1,1,2‐TCE), 1,1,2,2,‐tetrachloroethane (1,1,2,2‐TCE), pentachloroethane (PCE), 1,2‐dibromoethane (1,2‐DBE), iodoethane (IE), 1, 1‐dichloroethane (1,1‐DCE), 1,2‐dichloroethane (1,2‐DCE), 1,1,1‐trichloroethane (1,1,1‐TCE), 1,1,2‐trichloroethane (1,1,2‐TCE), dichloromethane (DCM), 1,1‐dichloroethylene (1,1‐DCEE) and trans 1,2‐dichloroethylene (t 1,2‐DCE) was administered (i.p.) in a dose‐response fashion (from 50 to 6.25% of the respective LD50) for three consecutive days, to Swiss Albino CD1 mice and the dealkylation of pentoxyresorufin examined in hepatic microsomes as 2B1 probe. For each hydrocarbon, linear regression was performed for both the inductive and toxic slope related to 2Bl‐like activity in order to evaluate the IC100 [the dose, in mmol/kg, pro...