Correlation Between MMP-7 and bFGF Expressions in Non-small Cell Lung Cancer Tissue and Clinicopathologic Features.

Abstract

The present study intends to investigate the correlation between clinicopathologic features of non-small cell lung cancer and matrix metalloproteinase-7 (MMP-7) and basic fibroblast growth factor (bFGF) and to investigate the roles of MMP-7 and bFGF in detecting the course of disease of non-small cell lung cancer. Ninety cases of paraffin-embedded tissue samples from patient with primary non-small cell lung cancer and fifty cases of lung tissue samples from normal subjects were included in the present study. Immunohistochemical S-P method was used to detect proteins MMP-7 and bFGF. (1) The positive rate of MMP-7 protein was 14% in normal lung tissue section and 68.89% in non-small cell lung cancer tissue section, and the difference was statistically significant (χ (2)=38.774, P=0.000<0.05). There were 43 cases (43/56) with positive expression in patients with squamous cell carcinoma and 22 cases (22/34) with positive expression in patients with adenocarcinoma, and the difference was not statistically significant (χ (2)=1.539, P=0.215>0.05). There were 14 cases (14/51) with positive expression in patients with moderate- and well-differentiated lung carcinoma and 36 cases (36/39) with positive expression in patients with poor-differentiated lung carcinoma, and the difference was statistically significant (χ (2)=35.068, P=0.000<0.05). There were 37 cases (37/42) with positive expression in patients with lymphatic metastasis and 26 cases (26/48) with positive expression in patients without lymphatic metastasis, and the difference was statistically significant (χ (2) =12.279, P=0.000<0.05). (2) The mean intratumoral microvessel density (iMVD) was 46.2±6.77 in the field of lung cancer tissue at high magnification under MMP-7-positive condition and 30.8±7.54 in the field of lung cancer tissue at high magnification under MMP-7-negative condition, and the difference was statistically significant (t=9.641, P=0.000<0.05). (3) The positive rate of bFGF was 12% in normal tissue section and 63.3% in non-small cell lung cancer tissue section, and the difference was statistically significant (χ (2)=34.222, P=0.000<0.05). There were 41 cases (41/56) with positive expression in patients with squamous cell carcinoma and 20 cases (20/34) with positive expression in patients with adenocarcinoma, and the difference was not statistically significant (χ(2)=2.006, P=0.157>0.05). There were 29 cases (29/51) with positive expression in patients with moderate- and well-differentiated lung carcinoma and 35 cases (35/39) with positive expression in patients with poor-differentiated lung carcinoma, and the difference was statistically significant (χ (2)=10.085, P=0.001<0.05). There were 37 cases (37/42) with positive expression in patients with lymphatic metastasis and 25 cases (25/48) with positive expression in patients without lymphatic metastasis, and the difference was statistically significant (χ (2) =13.554, P=0.001<0.05). (4) The (iMVD) was 45.8±7.16 in the field at high magnification under bFGF-positive condition and 31.2±6.46 in the field at high magnification under bFGF-negative condition, and the difference was statistically significant (t=9.654, P=0.001<0.05). (5) A correlation was demonstrated between MMP-7 and bFGF in non-small cell lung cancer (r=0.353, P=0.000<0.05). Both MMP-7 and bFGF are participated in the progression of non-small cell lung cancer and exert a synergistic effect during physiological processes including pathogenesis, invasion, and metastasis of non-small cell lung cancer. Therefore, a combined detection of MMP-7 and bFGF for non-small cell lung cancer contributes to predict the progression and prognosis of non-small cell lung cancer, with significant clinical value.