Abstract
One of the most significant indicators affecting male fertility is the sperm nuclear and mitochondrial DNA fragmentation index (DFI). DNA damage depends on biotic and abiotic factors, leading to oxidative stress (O.S.). This research aimed to investigate the relationship between mitochondrial dysfunction of spermatozoa and their biological adequacy. The research material was frozen-thawed sperm samples from the Ayrshire, Russian Black Pied Holstein, Russian Red Pied Holstein, Limousin, and Polled Russian breeding bulls. Assessments of mobility, morphology, and fragmentation index were performed using computer-assisted sperm analysis (CASA). It was found that there is a negative correlation between sperm activity and mitochondrial dysfunction with the correlation coefficient r = -0.24. The incidence of abnormal spermatozoa correlated with sperm dysfunction r = 0.77. The nDNA fragmentation index in chromatin varied from 0 to 25%.
Highlights
The sustainability and economics of bovine husbandry depend on obtaining high conception rates through artificial insemination (A.I.)
The success of A.I. and optimal use of genetically superior bulls is determined by the bull's fertility, which in turn depends on sperm quality in frozen-thawed semen doses [1]
This research aimed to study the relationship between mitochondrial dysfunction of spermatozoa and their biological adequacy
Summary
The sustainability and economics of bovine husbandry depend on obtaining high conception rates through artificial insemination (A.I.). The success of A.I. and optimal use of genetically superior bulls is determined by the bull's fertility, which in turn depends on sperm quality in frozen-thawed semen doses [1]. Sperm fertilizing potential is determined by their ability to reach the oocyte, complete fertilization, and sustain embryogenesis, partly determined by the quality of sperm DNA [1,2]. The accurate prediction of bull fertility is of primary economic importance in the Russian breeding industry. According to the research data, 20% of breeding bulls are infertile, and about 40% have reduced fertility [3]. The assessment of the biological adequacy of sperm is of great economic and biological importance
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