Abstract

Forty-one microsatellite markers were selected to analyze the genomic DNA of 68 progenies derived from a family of frost-resisted mirror carp German species. The results showed that a total of 100 different alleles were found, and the number of alleles in each locus was 2 to 4. The DNA fragment length was 91 bp to 376 bp, and the number of mean valid alleles was 1.428 7 to 3.945 4. The value of observed heterozygosity was 0.367 6 to 1.000 0, expected heterozygosity was 0.302 3 to 0.752 1 and the mean polymorphism information content (PIC) was 0.405 9. A GLM procedure was used to analyze the effects of these 41 microsatellites on body weight, height, head length and upper jaw length, followed by the outcome that marker hlj354 had a significant impact on body weight, length and head length; hlj855 on body weight, height and upper jaw length; hlj919 and hlj585 on head length. hlj895 and hlj704 on upper jaw length.

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