Correlation between low tapasin expression and impaired CD8+ T‑cell function in patients with chronic hepatitisB.

Abstract

Recent studies have demonstrated that chronic hepatitisB virus (HBV)infection is associated with reduced antigen‑presenting capacity and insufficient cytotoxic Tlymphocyte (CTL) production. The molecular chaperone tapasin mediates binding of the transporter associated with antigen processing (TAP), and has an important role in endogenous antigen processing and presentation, and the induction of specific CTL responses. The present study aimed to determine whether tapasin is associated with chronic HBV (CHB) infection. The mRNA expression levels of tapasin were detected in peripheral blood mononuclear cells from 27patients with CHB, 20patients with acute HBV (AHB) and 26healthy controls by reverse transcription‑quantitative polymerase chain reaction. In addition, CD8+ Timmune responses were evaluated in all groups, and the correlation between tapasin expression and CD8+ responses was analyzed. The results demonstrated that the mRNA expression levels of tapasin were significantly downregulated in patients with CHB compared with in healthy controls and patients with AHB. Furthermore, the apoptotic rate of CD8+ Tcells was increased in patients with CHB compared with in the other two groups. The percentage of interferon (IFN)‑γ+CD8+ Tcells was reduced in patients with CHB compared with in patients with AHB and healthy controls, and serum cytokine levels (IFN‑γ, interleukin‑2 and tumor necrosis factor‑α) were generally low in patients with CHB. Furthermore, the mRNA expression levels of tapasin were positively correlated with IFN‑γ production by CD8+ Tcells, and were inversely correlated with the apoptotic ratio of CD8+ Tcells. These results indicate that decreased expression of tapasin may be closely associated with CHB, and suggest an important role for tapasin in the pathogenesis of CHB.