Abstract
Temperature, which is an important environmental factor in broiler farming, can significantly influence the deposition of fatty acids in muscle. 300 one-day-old broiler chicks were randomly divided into three groups and reared at high, medium and low temperatures (HJ, MJ and LJ), respectively. Breast muscle and jejunal chyme samples were collected and subjected to analyses of fatty acid composition and 16S rRNA gene sequencing. Through spearman’s rank correlation coefficient, the data were used to characterize the correlation between jejunal microbial diversity and muscle fatty acid deposition in the broilers. The results showed that Achromobacter, Stenotrophomonas, Pandoraea, Brevundimonas, Petrobacter and Variovorax were significantly enriched in the MJ group, and all of them were positively correlated with the fatty acid profiling of muscle and multiple lipid metabolism signaling pathways. Lactobacillus was significantly enriched in the HJ group and exhibited a positive correlation with fatty acid deposition. Pyramidobacter, Dialister, Bacteroides and Selenomonas were significantly enriched in the LJ group and displayed negative correlation with fatty acid deposition. Taken together, this study demonstrated that the jejunal microflora manifested considerable changes at high and low ambient temperatures and that jejunal microbiota changes were correlated with fatty acid deposition of muscle in broilers.
Highlights
Findings demonstrated that the microbiome in jejunum may be a highly relevant target in our efforts to solve the problems associated with fat deposition
This study aims to explore the links of microbial diversity and fatty acid composition in broilers raised at different ambient temperatures, thereby providing experimental evidence to understand on how environmental temperatures affect the health and growth performance of broilers via modulation of intestinal microbiota
The proximal small intestine serves as the major region of macronutrient digestion and absorption, whereas jejunum is a structurally and functionally distinct region of the alimentary tract that connects the upper portion of the small intestine to the lower portion of the small intestine
Summary
Findings demonstrated that the microbiome in jejunum may be a highly relevant target in our efforts to solve the problems associated with fat deposition. Significant shifts in proportions of fecal microbial population were seen on mice when maintained under the cold environment with higher ratio of Firmicutes/Bacteroidetes[13]. These studies suggest that changes in gut microbiome diversity and/or richness at varied environment temperatures have shown to be associated with fat acid deposition. Little is known about underlying mechanism that gut microbiota regulate the fatty acid metabolism in broilers at different environmental temperatures. This study aims to explore the links of microbial diversity and fatty acid composition in broilers raised at different ambient temperatures, thereby providing experimental evidence to understand on how environmental temperatures affect the health and growth performance of broilers via modulation of intestinal microbiota
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