Abstract
Male Wistar rats were subjected to septic shock by means of intravenous administration of lipopolysaccharide (LPS, 8 mg/kg). Blood samples were taken from the left carotid artery initially and 1.5 and 6 hours after LPS infusion. The blood samples were treated with heparin and centrifuged. The HbNO adduct was measured in the red cell fraction through electron paramagnetic resonance, while reduced nitrates (NOx) were measured in plasma (Griess method, Cayman Chem Co. kit). No HbNO was detected in the basal samples. When compared to the 1.5 h HbNO levels those at 6 hours significantly increased after LPS administration. The basal levels of NOx did not change after 1.5 h but significantly increased 6 h after LPS administration. A significant correlation between HbNO and NOx was only observed 6 hours after LPS administration. Since NO needs deoxyhemoglobin to form the HbNO adduct, it is concluded that a correlation between HbNO and NOx levels could be an indicator of hypoxia in septic shock. This study was supported by grants SIP20110949 and CONACYT083090
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