Correlation between expressions of IgE and CD23 and primary nephrotic syndrome in children

Abstract

Objective To explore the correlation between the expressions of IgE and CD23 and primary nephrotic syndrome in children.Methods 52 children with primary nephrotic syndrome and 13 healthy children were recruited into this study.The serum IgE concentrations were detected by immunoradiation assay.The proportion of CD23 positive cells were determined by flow cytometry analysis.Results The IgE concentration and the proportion of CD23+ cells in the children with primary nephrotic syndrome were significantly higher than those of the health counterparts (IgE:[452.5 ± 239.7] vs.[132.6 ± 105.5],P ＜ 0.01; CD23+ cells％:[16.6 ± 7.6] vs.[5.2 ± 3.9],P ＜ 0.05).The serum IgE concentration was obviously higher in the patients with steroid-resistant nephrotic syndrome (SRNS) than in the children with steroid-sensitive nephrotic syndrome (SSNS) before and after the treatment (before the treatment:[462.9 ± 321.2] vs.[382.1 ± 269.0],P ＜ 0.05; after the treatment:[246.7 ± 182.1] vs.[171.7 ± 107.9],P ＜ 0.05).The proportion of CD23+ cells was significantly enhanced in the SRNS children than in the SSNS children before and after the treatment (before the treatment:[19.3 ± 6.4] vs.[15.6 ± 7.7],P ＜ 0.05; after the treatment:[12.2 ± 8.5] vs.[6.1 ± 4.3],P ＜ 0.01).IgE concentration positively correlated with CD23+ proportion in the children with primary nephrotic syndrome (r=0.605,P ＜ 0.05).Conclusions Immune dysfunction was observed in children with primary nephrotic syndrome,as indicated by the abnormal expression of serum IgE concentration and CD23+ cell proportion.Compared to SSNS children,these disorders were more obvious in SRNS children befaore and after treatment.Therefore,a combined testing of serum IgE concentration and CD23+ cell proportion shows great significance in the diagnosis and prognosis evaluation in primary nephrotic syndrome in children. Key words: Primary nephrotic syndrome; children; IgE; CD23