Abstract
Ribosomal DNA (rDNA) polymorphism was compared to enzyme polymorphism and DNA/DNA hybridization data for the intraspecies differentiation of Providencia alcalifaciens. DNA from 27 strains previously classified into two zymotypes A 1 and A 2 and discriminated by two levels of DNA/DNA hybridization (ΔT m values of 0 to 1°C and 6 to 10°C, respectively) were analysed by Southern blotting for rDNA polymorphism. The ribotypes fell into two ribogroups A 1 and A 2, which correlated with the corresponding zymotypes. This correlation argues for the proposed creation of two species within P. alcalifaciens.
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