Abstract
ObjectiveThis study aimed to determine the relative amounts of Streptococcus mutans (SM) and lactobacilli (LBs) and their relationship with dental caries among a Yemeni adult population.ResultsA positive correlation appeared between SM and LB counts from saliva and caries tissue samples and the decayed, missing and filled teeth (DMFT) score (p < 0.05). There was a significant correlation between SM and LBs in caries tissue (p < 0.05). However, there was no significant difference between SM and LBs isolated from saliva samples (p > 0.05). The number of SM and LBs in subjects with active caries was significantly higher than that in those without active caries (p < 0.05). There was no significant difference between the daily habits and SM and LB or DMFT scores (p > 0.05), except for a significant difference between brushing frequency and DMFT score (p < 0.05).
Highlights
Dental caries is a major infectious disease affecting the majority of the world’s population [1]
For the construction of quantification standard curves, the Quantitative polymerise chine reaction (qPCR) reaction mixture contained a total volume of 20 μL, which consisted of 10 × Eva Green qPCR Mix Plus ROX (Solis Bio Dyne, Estonia), 2 μM each forward and reverse primer, 5 μL of template genomic DNA from the aforementioned bacterial strains and 3 μL DNA/RNAsefree water [14]
A positive correlation was found between Streptococcus mutans (SM) isolated from CA saliva and caries tissue samples and the DMFT score (p < 0.05), whereas no significant relationship was found between SM isolated from caries tissue and the DMFT score (p > 0.05)
Summary
Based on qPCR results, SM and LBs were detected in 100% of the Yemeni adults (Fig. 1; Additional files 3 and 4). No significant relationship was found between LBs isolated from saliva and caries tissue samples with the DMFT score (p > 0.05). There was a significant inverse correlation between SM and LBs isolated from caries tissue (p < 0.05) 0.174 1.00 0.833 levels and DMFT scores (p > 0.05), except for brushing frequency and DMFT scores, which showed a significant difference between the two groups (p < 0.05) (Additional file 5)
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