Abstract

ABSTRACT Photodynamic therapy (PDT) can be target ed toward different subce llular localizations and it is widely believed different subcellular targets vary in their sensitivity to photobiological damage. In this study, PDT-ge nerated near-infrared singlet oxygen ( 1 O 2 ) luminescence was measured along with cell viability under two different incubation protocols: 5-aminolevulinic acid (ALA) endogenously-induced protoporphyrin IX (PpIX) and exogenous PpIX, at different incubation times. Confocal fluorescence microscopy indicated that ALA-induced PpIX (2 h) localized in the mitochondria, whereas exogenous PpIX (1 h) mainly localized to the plasma membrane. Cell viability was determined at several time points during PDT treatments using colony-forming assays, and the surviving fraction correlated well with cumulative 1 O 2 luminescence counts under both incubation protocols. Preliminary results indicate the plasma membrane is less sensitive to PDT-generated 1 O 2 than the mitochondria. Keywords: Photodynamic therapy, ALA, PpIX, Subcellular localization, Singlet oxygen, Cell viability

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