Abstract

The simultaneous determination of the cell cycle phase of individual adherent Chinese hamster ovary cells using a fluorescence microscope after staining with 4',6-diamidine-2'-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by a phase-shifting laser microscopy revealed that the laser phase shift of cells in the G2/M phase was markedly higher than that of cells in the G1 and S phases.

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