Correlation between alanine inhibition of pyruvate kinase and composition of K-M hybrids

Abstract

A method is described for determination of the percentage of K-subunits in the K-M set of pyruvate kinase. The method is based on the alanine inhibition of the K-subunits. A linear correlation ( r = 0.99) is found between the alanine inhibition and the percentage of K-subunits as calculated from the electropherograms, assuming (a) a subunit distribution as indicated by the suffix in K 4, K 3M, K 2M 2, etc., and (b) an equal contribution of K- and M-subunits to the intensity of the stain.