Correlation between 50-kHz band activity in primary auditory cortex and social interaction in rats

Abstract

Event Abstract Back to Event Correlation between 50-kHz band activity in primary auditory cortex and social interaction in rats Hengjun J Kim1*, Gyunggoo Cho1, Youngkyu Song1, Song-I Chun1, Dongwan Lim1, Jeong Kon Kim2 and Young Ro Kim3 1 Korea Basic Science Institute, Korea 2 Radiology, Asan medical center, Korea, Republic 3 Radiology, Massachusetts General Hospital, United States Ultrasonic vocalization (USV) reflects emotional state and the level of social interaction in rats. The USV in 50-kHz band are emitted in a positive affective state induced by food reward or rough-and-tumble play as well as by the approach behavior, whereas 22-kHz band relates to an anxious state. Primary auditory cortex (Aud1) of rat is tonotopic, in which spatial divisions of Aud1 corresponds to specific frequency bands. Based on the work of Rutkowski et al. (2003), we created probabilistic maps to display the 50-kHz or 22-kHz band responsivity in Aud1 (Figure A). Penetrating through the blood-brain barrier, manganese ions (Mn2+) enter and accumulate in the firing neurons via voltage-gated calcium channels, enhancing the MRI signal intensity. Thus, we applied manganese-enhanced magnetic resonance imaging (MEMRI) to investigate the relation between social behavior and 50-kHz band activity in Aud1 using MDEFT pulse sequence (TR/TE=15/3.8ms, Inversion delay=1100ms, 4 segments, FA=15°) and social interaction test (SIT). First, SIT was performed based on File et al. (1978)'s protocol. Female pairs of rats (6 pairs, n=12) were placed in the box for 10 min and SIT score was measured by the time spent in active social contacts including sniffing, grooming, and following. Secondly, we acquired high-resolution image before and after Mn2+ injection to rats to monitor the brain activity for one day. From the brain activity map and probabilistic map, we calculated Aud1 activities in 50-kHz band region, which were correlated with SIT score. Figure B showed the relation between brain activities and SIT score for left and right Aud1 regions. Only in the left Aud1, the neural activities receiving 50-kHz band had significant correlation with the SIT score (r=0.70, p=0.01), indicating that animals showing active social behavior had enhanced neural activities to 50-kHz calls in Aud1. Previous behavioral studies showed that the dominant rat had higher SIT score than subordinates, and high SIT score was known to represent low anxiety level. Our result revealed that the animals with low anxiety level had positive bias in the perception of ultrasonic calls. In addition, we suggest that the probabilistic map, if it was applied to functional brain imaging data, could be used to measure the affective valence in the auditory sensory cortex. References RG Rutkowski et al. (2003) Hear. Res. 181, 116-130. SE File et al. (1978) Br. J. Pharmacol. 62, 19-24. Figure 1 Keywords: Neuroimaging, primary auditory cortex, social interaction in rats, manganese ions, voltage-gated calcium channels Conference: 5th INCF Congress of Neuroinformatics, Munich, Germany, 10 Sep - 12 Sep, 2012. Presentation Type: Poster Topic: Neuroinformatics Citation: Kim H, Cho G, Song Y, Chun S, Lim D, Kim J and Kim Y (2014). Correlation between 50-kHz band activity in primary auditory cortex and social interaction in rats. Front. Neuroinform. Conference Abstract: 5th INCF Congress of Neuroinformatics. doi: 10.3389/conf.fninf.2014.08.00004 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Mar 2013; Published Online: 27 Feb 2014. * Correspondence: Dr. Hengjun J Kim, Korea Basic Science Institute, Daejeon, Korea, heyjoon73@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hengjun J Kim Gyunggoo Cho Youngkyu Song Song-I Chun Dongwan Lim Jeong Kon Kim Young Ro Kim Google Hengjun J Kim Gyunggoo Cho Youngkyu Song Song-I Chun Dongwan Lim Jeong Kon Kim Young Ro Kim Google Scholar Hengjun J Kim Gyunggoo Cho Youngkyu Song Song-I Chun Dongwan Lim Jeong Kon Kim Young Ro Kim PubMed Hengjun J Kim Gyunggoo Cho Youngkyu Song Song-I Chun Dongwan Lim Jeong Kon Kim Young Ro Kim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.