Abstract

The research aim is a correlation analysis of the PRM1, STK35 and IFT27 genes mRNA expression with the native and decryoconserved sperm quality indicators of Holstein bulls to search for effective transcriptomic biomarkers of bull semen. In the study course, native and decryoconserved sperm of seven Holstein bulls were used. To solve the study problems, eight indicators of sperm quality were studied, the studied genes in native and decryoconserved spermatozoa expression was analyzed in real time PCR. Nonparametric probabilistic and statistical methods were used, the analysis of rank correlation was carried out using Spearman’s criterion. Higher expression of the studied genes was mainly noted in frozen--thawed sperm compared to native. The mRNA expression level of the protamine gene (PRM1) did not give a reliable correlation with sperm parameters: The level ITF27 gene mRNA expression was significantly positively correlated with the content of defective cells from frozen--thawed sperm (0.714, p=0.05) and dead cells (0.714, p=0.0545) from native sperm. A negative correlation was noted with the content of normal cells in frozen--thawed sperm (-0.750, p=0.038) and live cells (-0.714, p=0.0545) in native sperm. The ITF27 gene transcript (mRNA) showed a negative correlation (-0.703, p=0.0545) in terms of the acrosome defect of frozen--thawed spermatozoa. In terms of the content of reactive oxygen species (ROS), the mRNA of the ITF27 gene had a significant positive correlation (0.786, p=0.0251). The STK35 gene transcript (mRNA) was the only one of all the studied mRNAs that had an average negative correlation with sperm motility in native (-0.692, p=0.052) and frozen--thawed sperm (-0.876, p=0.035). These studies can be used to create a system of non-invasive transcriptional markers of bull sperm quality.

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