Correlation analysis of surfactant protein-C genetic polymorphisms and neonatal respiratory distress syndrome of the Mongol nationality in Inner Mongolia

Abstract

Objective To investigate the distribution of surfactant protein-C (SP-C) gene single nucleotide polymorphisms and to study the association between the SP-C gene polymorphisms and neonatal respiratory distress syndrome (NRDS) in infants. Methods Fifty-one infants with NRDS (NRDS group) and 51 infants without RDS (control group) were selected. PCR gene analysis and polymerase chain reaction were used to establish the genotype and allele frequencies of SP-C exon 4(T138N) and exon 5(S186N), SP-C exon 4 and 5 for the mutation, and then the association between the polymorphisms and NRDS was analyzed. Results SP-C gene mutations were not found in exon 4 and 5. In the Mongol nationality of the Inner Mongolia region, SP-C exon 4(T138N) genotypes could check out three genotypes: namely AA, AC and CC. The frequencies of allele A and allele C of SP-C exon 4(T138N) were not statistically different between NRDS group and control group (χ2=0.454, P=0.797). In the Mongol nationality, SP-C exon 5(S186N) genotypes could check out three genotypes: namely AA, AG and GG. The frequencies of allele A and allele G of SP-C exon 5(S186N) were not statistically different between NRDS group and control group (χ2=0.493, P=0.782). Conclusion SP-C exon 4(T138N) and exon 5(S186N) gene polymorphism in Inner Mongolia newborns displays no significant correlation with sex, birth weight or gestational age. SP-C gene mutations are not found in exon 4 and 5. SP-C gene exon 4(T138N) and exon 5(S186N) polymorphisms are not found to be associated with NRDS in Mongol nationality of the Inner Mongolia. Key words: Respiratory distress syndrome; Surfactant protein C; Gene polymorphisms; Newborn