Correlation analysis of positive rate of HPV genotyping test and HPV nucleic acid loads

Abstract

Objective To investigate the correlation between the positive rate of PCR-reverse dot blot genotyping test and the loads of the viral nucleic acid. Methods The fluorescent PCR assay was used to detect the high-risk HPV（HR-HPV）DNA loads in the cervical mucus samples from 1162 female patients. Patients with positive HR-HPV DNA were further analyzed by PCR-reverse dot blot hybridization assay for HPV genotyping. Results The overall positive rate of genotyping test was 68. 8% . The positive rate of genotyping test had a significant positive correlation with the Log Koc of HR-HPV DNA loads（r=0. 944, P﹤0. 01）. The quadratic curve fitting formula was Y= -1. 806＋0. 558X-0. 031X2（Y for genotyping positive rate,X for Log Koc of HR-HPV DNA loads）. There were significant differences with the positive rate of genotyping test among patients with different viral loads（P﹤0. 01）. When HR-HPV DNA loads were 10^4-10^5 copies/ ml,10^5-10^6 copies/ ml,10^6-10^7 copies/ ml and more than 10^7 copies/ ml,the positive rate of HPV genotyping test were 27. 8% ,48. 5% ,74. 0% ,97. 5% and 33. 3% ,51. 5% ,78. 0% ,97. 5% respectively by using different genotyping detection reagents. Conclusion The positive rate of PCR-reverse dot blot genotyping test was correlated with the loads of HPV nucleic acid. Key words: Human papillomavirus; Viral load; Polymerase chain reaction; Genotyping; Reverse dot blot