Correlation analysis in liquid chromatography of metal chelates : II. One-dimensional retention-mobile phase composition (physicochemical property) models in reversed-phase liquid chromatography

Abstract

The applicability of linear correlation models for describing the effect of mobile phase composition and properties on the retention of metal chelates in reversed-phase liquid chromatography is demonstrated. The macroscopic (dielectric constant, surface tension, viscosity, concentration of organic modifier, distribution constant in an n-octanol-water system, etc.) and microscopic (characteristics of donor and acceptor ability) parameters of binary water-organic mixtures are used as variables. Although each of the studied parameters alone is somewhat limited in application, together they allow retention values to be estimated and the chromatographic behaviour of metal chelates to be predicted. The dominant influence of mobile phase composition and proton-donating ability on chelate retention in reversed-phase liquid chromatography is confirmed.