Abstract
BackgroundOsteonecrosis of the femoral head (ONFH) is a complicated disease associated with trauma, hormone abuse and excessive alcohol consumption. Polymorphisms of long non-coding RNAs have been also linked with the development of ONFH. Our research aimed to explore the relationship between CARMEN (Cardiac Mesoderm Enhancer-Associated Non-Coding RNA) variants and ONFH risk.MethodsOur study used Agena MassARRAY Assay to genotype 6 selected single nucleotide polymorphisms (SNPs) in 731 participants (308 alcohol-induced ONFH patients and 423 controls). We used odds ratios (ORs) and 95% confidence intervals (CIs) to calculate the effect of gene polymorphisms on the occurrence of alcohol-induced ONFH by logistic regression analysis and haplotype analysis.ResultsOur overall analysis illustrated that rs13177623 and rs12654195 had an association with a reduced risk of ONFH after adjustment for age and gender. We also found that rs13177623, rs12654195 and rs11168100 were associated with a decreased susceptibility to alcohol-induced ONFH in people ≤45 years. In addition, the necrotic sites stratification analysis showed that rs12654195 was only found to be related to alcohol-induced ONFH risk in the recessive model. In patients with different clinical stages, rs353300 was observed to be associated with a higher incidence of ONFH. Individuals with different genotypes of rs13177623, rs12654195 and rs11168100 had significantly different clinical parameters (cholinesterase, globulin, percentage of neutrophils and the absolute value of lymphocytes).ConclusionsOur data provided new light on the association between CARMEN polymorphisms and alcohol-induced ONFH risk in the Chinese Han population.
Highlights
Osteonecrosis of the femoral head (ONFH) is a complicated disease associated with trauma, hormone abuse and excessive alcohol consumption
Osteonecrosis of the femoral head (ONFH) is a devastating orthopedic disease, which is characterized by bone cell death due to the damage of microvascular circulation, which is considered to be the result of mechanical vascular disruption, intravascular occlusion and extravascular compression [1, 2]
Of population recruited from Department of Orthopedics of Zhengzhou Chinese Hospital, the mean age of 308 cases and 423 controls were 43.47 ± 11.303 years and 42.52 ± 13.135 years, respectively
Summary
Osteonecrosis of the femoral head (ONFH) is a complicated disease associated with trauma, hormone abuse and excessive alcohol consumption. Polymorphisms of long non-coding RNAs have been linked with the development of ONFH. Our research aimed to explore the relationship between CARMEN (Cardiac Mesoderm Enhancer-Associated Non-Coding RNA) variants and ONFH risk. ONFH usually occurs in people aged 30–50 years and this refractory disease has a high disability rate [3]. It is estimated that there are 8.12 million ONFH cases in Chinese people aged 15 years and over [6]. Increasing evidence showed that ONFH is a complex disease associated with many factors, such as trauma [7], genetic factors [8], high-dose corticosteroid use [9] and excessive alcohol consumption. Thereinto, genetic polymorphisms of some genes played critical roles in the occurrence of alcohol-induced ONFH [12,13,14,15,16,17], such as MMP20, RETN, ApoB, ApoA1, NOS3
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