Abstract

AbstractThe development of diffraction-unlimited fluorescence microscopy has paved the way for a detailed understanding of cellular dynamics since live-cell investigations with resolutions below 100 nm have become available. This allows studying labelled specimen such as proteins and their distribution within a cell at a new level of detail. However, examining the cell membrane as a site of interaction between the cell’s internal processes and external signals with fluorescence microscopy (FM) leads to the difficulty that certain types of fluorescent dyes can alter the physicochemical properties of a cell. Investigating the cell membrane in a contact-free way and without any labelling or special pre-treatment is one of the greatest strengths of scanning ion conductance microscopy (SICM), which uses a fine pipette tip to determine the specimen’s topography. Since FM and SICM can both be applied to living cells and provide a resolution below the diffraction limit, correlating data from these two techniques seems to be a promising approach to further the understanding of the cell membrane’s organization and the mechanisms involved in transport processes across it. In the following, we briefly introduce the different approaches to super-resolved fluorescence microscopy (SRFM) and review applications of correlating SICM and FM (diffraction-limited) as well as SICM and SRFM (diffraction-unlimited). Finally, we discuss potential implementations of combined SRFM/SICM instruments and pitfalls that may arise during the development of such instruments.KeywordsCorrelative microscopyDiffraction-unlimited fluorescence microscopyPhotoactivated localization microscopy (PALM)Scanning ion conductance microscopy (SICM)Stimulated emission depletion (STED) microscopySuper-resolution fluorescence microscopySuper-resolved fluorescence microscopy

Full Text

Published Version

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.