Abstract
In combination with laser scanning microscopes, optical imaging technologies based on time correlated single photon counting (TCSPC) are successfully used in fluorescence lifetime imaging microscopy (FLIM) providing monitoring of intracellular intrinsic metabolic coenzymes as NAD(P)H (nicotinamide adenine dinucleotide (phosphate)). Due to oxygen-dependent quenching of the phosphorescence of some compounds including transition metal complexes, the phosphorescence lifetime imaging microscopy (PLIM) can be used for evaluation of oxygen partial pressure (pO2). Using a multi-channel FLIM/PLIM system, we were able to monitor pO2 by PLIM simultaneously with NAD(P)H by FLIM providing complex metabolic and redox imaging of living cells and tissues.
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