Abstract
[This corrects the article DOI: 10.1371/journal.pbio.1000521.].
Highlights
The authors have provided a corrected version here
To allow for simultaneous detection of proteins of different molecular weights, membranes were at times cut horizontally approximately 25 kD below and above the expected molecular weight, prior to incubation with antibodies
For IP experiments that test the binding between immunoprecipitated and co-precipitated proteins during different conditions, blotting is required on the same filter to ensure that the loading does not impact the interpretation of the results
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