Correction of vaginal dysbiosis with the use of probiotics

Abstract

Disbacteriosis of open cavities of the human body ic connected with the formation of opportunistic bacteria dominance in the composition of the microflora, displacing the representatives of normal microflora which leads to disorders and aggravation of health. Among serious consequences of vaginal dysbiosis are increased risk of infection with pathogens through lack of effect of colonization resistance; in some cases it may result in pregnancy loss and infertility. The most common vaginal dysbiosis forms are due to predominance of staphylococci and enterobacteria, that actively colonize the lower genital tract. Therefore, disbacteriosis needs a correction for which antibiotics and probiotic preparations are used. Sometimes antibiotics are not effective, so the correction scheme is required which would be highly efficient in restoring the microflora composition. The aim of research was to investigate the efficacy of scheme of sequential use of two probiotic preparations, based on spore bacilli and lactobacilli. The method of correcting the microbial vaginal ecosystem of mice with experimental dysbiosis with the use of two-stage administration of probiotic preparations (biosporin and vagilak) was proposed. One of these preparation contains spore bacilli with high antagonistic potential, and the second one contains lactobacilli which are typical representatives of this habitat. The scheme was tested in the experimental model of vaginal dysbiosis of mice, created by intravaginal administration of cell suspension of Staphylococcus aureus culture. Cell suspension of probiotic microorganism cultures was administered intravaginally to animals, each preparation for 5 days in succession. It was found that the most effective correction of dysbiosis occurred by sequential use of Biosporin and Vagilak preparations: full renovation of vaginal microflora was recorded for 20 days from the start of probiotics use, that as, 5 day faster than when following the standard schemes, including antibiotic and probiotic. It was shown that renewal of vaginal microflora of mice was expressed in increasing number of lactobacilli 2.88 times returning to the level of control for the 20th day, reducing the number of staphylococci and enterobacteria 2.81 and 5.13 times, respectively, on the 10th day of the experiment, as well as increasing the ratio of aerobes/anaerobes – 1/58 to that of the control group – 1/60. The results demonstrated the benefits of high efficiency of the proposed scheme, which allowed to recommend this method in further research to develop a framework for practical use.