Correction: Modulation of Malaria Infection in Anopheles gambiae Mosquitoes Exposed to Natural Midgut Bacteria

Majoline T Tchioffo,Richard Christen,Parfait H Awono-Ambã©Nã©,Luc Abate,Geoffrey Gimonneau,Sandrine E Nsango,Antoine Berry,Isabelle Morlais,Thomas S Churcher,Anne Boissiã¨Re

doi:10.1371/annotation/d8908395-a526-428c-b9ed-4430aaf8f7d7

Abstract

There were multiple errors in the second paragraph of the Effect of bacterial challenge on P. falciparum development section of the Results: When efficacy was measured as changes in oocyst intensity, the bacterial challenge had no effect irrespective of the oocyst intensity in the control group (Figure 3C). However, the reduction in oocyst prevalence due to the bacterial exposure was lower when parasite exposure was higher – in feedings that gave rise to the highest oocyst intensities in the PBS control, the bacterial challenge had almost no effect (Figure 3B). In addition, an affiliation of the ninth author is missing: In addition to institution number 7, Antoine Berry is also affiliated with the the following institution: Centre de Physiopathologie de Toulouse-Purpan, Institut National de la Sante et de la Recherche Medicale, UMR1043, CNRS UMR5282, Universite de Toulouse Paul Sabatier, Toulouse, France.

Highlights

Malaria remains a major public health burden in developing African countries, with approximately 1 million malaria deaths were reported in 2010 alone [1]
We have previously shown that the composition of the midgut microbiota is a major component that determines mosquito competence, and by contrast to co-infection studies we found in field mosquitoes with native midgut microbiota that the abundance of Enterobacteriaceae was positively correlated with the P. falciparum infection [11]
The 16S rRNA gene sequences of the bacteria isolated from the mosquito midgut were aligned with published sequences of bacterial strains described in other insect vectors

Summary

Introduction

Malaria remains a major public health burden in developing African countries, with approximately 1 million malaria deaths were reported in 2010 alone [1]. In Sub-saharan Africa, Plasmodium falciparum is responsible for the devastating impact of the disease and Anopheles gambiae is its main mosquito vector. Co-infections of bacteria with P. falciparum exhibited a reduced number of developing oocysts in the mosquito midgut, both in laboratory and field studies [5,13,14,15,16,17,20,21]. It has been suggested that the interaction between commensal bacteria and the mosquito epithelial cells invaded by P. falciparum would elicit immune responses leading to reduced levels of parasite burden, though it could be due to bacteria producing toxins with anti-Plasmodium activity [8,14,22]

Methods

Results

Conclusion