Correction for Pozsgai et al., "Modified mariner Transposons for Random Inducible-Expression Insertions and Transcriptional Reporter Fusion Insertions in Bacillus subtilis".

Abstract

Volume 78, no. 3, p. 778–785, 2012, https://doi.org/10.1128/AEM.07098-11. The TnKRM series of transposons functions as advertised in Bacillus subtilis, but the delivery plasmids carrying the transposons are unstable when propagated in Escherichia coli. We recommend one of three solutions. The first option is to provide (i) the plasmid carrying the delivery system and mariner transposase and (ii) another plasmid carrying the TnKRM transposon separately such that a laboratory can reconstitute the entire system by cloning de novo and thereby minimize E. coli propagation. The second option is to provide phage lysates grown on B. subtilis strains carrying a functional TnKRM delivery plasmid that can be used to transfer the plasmid into the B. subtilis strain of interest by SPP1-mediated generalized transduction. As a third option, we have created an entirely new transposon system, TnFLX, using a low-copy-number delivery plasmid for increased stability in E. coli as described in this issue (F. Dempwolff, S. Sanchez, and D. B. Kearns, Appl Environ Microbiol 86:e02893-19, 2020, https://doi.org/10.1128/AEM.02893-19).