Abstract
There was an error in the corresponding author designation. Bosiljka Tasic and Liqun Luo are both corresponding authors. Bosiljka Tasic's e-mail address is bosiljka.tasic@gmail.com. Liqun Luo's published e-mail address was incorrect; lluo@stanford.edu is correct.
Highlights
Mosaic animals contain cells with different genotypes
Design of new Mosaic Analysis with Double Markers (MADM) cassettes The original version of MADM relied on the DsRed2 fluorescent protein as one of the two markers [10]
The new TG cassette is compatible with any GFPN-terminus-intron-XATG-less cassette, where XATG-less is any gene without the start codon (Figure 1B, bottom right)
Summary
Mosaic animals (genetic mosaics) contain cells with different genotypes. Phenotypic analysis of genetic mosaics has become an indispensible tool in modern genetics. Interchromosomal recombination techniques depend on recombination between chromatids after DNA replication in the G2 phase of the cell cycle to generate sibling cells of different genotypes. The common and key feature of these approaches is that they create cells with different genotypes in vivo and at the same time label those cells with unique markers that strictly correlate with the genotype. To enable such concomitant in vivo genetic manipulation and labeling in mammals, we have established Mosaic Analysis with Double Markers (MADM) in mice (Figure 1A) [10]. To expand the utility and versatility of MADM, we present here modifications and new applications of the technique, and compare different procedures for establishment of MADM-ready chromosomes
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