Correction: Engineering of Recombinant Poplar Deoxy-D-Xylulose-5-Phosphate Synthase (PtDXS) by Site-Directed Mutagenesis Improves Its Activity.

Aparajita Banerjee,Thomas D Sharkey,Alyssa L Preiser

doi:10.1371/journal.pone.0165028

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0161534.].

Highlights

Zoomed in surface view of the orientation of Ala-147 residue of WTPtDXS and the thiazolium ring of ThDP in the enzyme active site.
B. Cartoon view of the interactions of different residues of WTPtDXS with ThDP and their relevant distances from the thiazolium ring and the carbon chain of ThDP.
S2 Fig. SDS-PAGE of the different fractions from the Ni-NTA column purification of recombinant WT and the various mutants of PtDXS.

Summary

Introduction

Zoomed in surface view of the orientation of Ala-147 residue of WTPtDXS and the thiazolium ring of ThDP in the enzyme active site. B. Cartoon view of the interactions of different residues of WTPtDXS with ThDP and their relevant distances from the thiazolium ring and the carbon chain of ThDP.

Results

Conclusion